Table 2 Oligonucleotide primers used for PCR reactions
From: Alterations of DNA damage-response genes ATM and ATR in pyothorax-associated lymphoma
1st primer sequence | 2nd primer sequence | PCR products (bp) a | |
|---|---|---|---|
cDNA primers | |||
ATM1 | F 5′-CTGCTGCCCAGATATGACTTC-3′ | 5′-CAGTGATGTGTGTTCTGAAATTGTG-3′ | 2113 (nt. 161–2273)b |
R 5′-AGAAGACAGCGATCCAGTG-3′ | 5′-AGAAGACAGCGATCCAGTG-3′ | ||
ATM2 | F 5′-GTTACGATGCCTTACG-3′ | 5′-GGCTTCTCTGTCCACCAG-3′ | 2316 (nt. 2224–4539) |
R 5′-CAGTAATAAACTAACAAACAGGTG-3′ | 5′-CAGTAATAAACTAACAAACAGGTG-3′ | ||
ATM3 | F 5′-AGAACCTCACCTTGTG-3′ | 5′-CAAATAATGTTTATAAGAAGCACAG-3′ | 1518 (nt. 4475–5992) |
R 5′-CCAGCCAGAAAGCATC-3′ | 5′-CCAGCCAGAAAGCATC-3′ | ||
ATM4 | F 5′-GCCTGGATGATATAAATC-3′ | 5′-GCTTGCTGTTGTGGAC-3′ | 1875 (nt. 5916–7790) |
R 5′-CCTCCCATCATCTTGGTCC-3′ | 5′-CCTCCCATCATCTTGGTCC-3′ | ||
ATM5 | F 5′-GATGAAGAGAGACGGAATGAAG-3′ | 5′-GATGAAGAGAGACGGAATGAAG-3′ | 1696 (nt. 7698–9393) |
R 5′-GTATGTTGGCAGGTTAAAAATAAAGGCT-3′ | 5′-GGCTGAATGAAAGGGTAATTCATATAC-3′ | ||
ATR1 | F 5′-GGAGACGCCGGGAAC-3′ | 5′-CGTTGGCGTGGTTGAC-3′ | 2109 (nt. 70–2179) |
R 5′-GAATCTTGGGAACTCTGTTAC-3′ | 5′-GAATCTTGGGAACTCTGTTAC-3′ | ||
ATR2 | F 5′-GCACTGAAACAGAAAGCTGA-3′ | 5′-GTGGAGAACAGCAGTTTAC-3′ | 2112 (nt. 2055–4166) |
R 5′-CAGAGCAACCGAGCTTG-3′ | 5′-CAGAGCAACCGAGCTTG-3′ | ||
ATR3 | F 5′-ATCACCTGAACTGATGGCT-3′ | 5′-CTGATAAAGTATGCAACAGAC-3′ | 2072 (nt. 4057–6128) |
R 5′-CGGCCCACTAGTAGCAT-3′ | 5′-CGGCCCACTAGTAGCAT-3′ | ||
ATR4 | F 5′-CACCAGGCACTAATTGTTC-3′ | 5′-CACCAGGCACTAATTGTTC-3′ | 2073 (nt. 6013–8085) |
R 5′-CAACCACAGATTCATACC-3′ | 5′-GCATTACTTTTAGATTATTAACAT-3′ | ||
Genomic primers | |||
ATMexon8 | F 5′-AAATCCTTTTTCTGTATGGGAT-3′ | 350 | |
ATMexon8 | R 5′-GTTACTGAGTCTAAAACATGG-3′ | ||
ATMexon33 | F 5′-ATTACAGTAAGTTTTGTTGGCT-3′ | 317 | |
ATMexon33 | R 5′-GCTAGAGCATTACAGATTTTTG-3′ | ||
ATMexon34 | F 5′-CAGTGTCTATAAATGGCAC-3′ | 324 | |
ATMexon34 | R 5′-GTGACAATGAAACCAAGAG-3′ | ||
ATMintron61 | F 5′-ATTTAATAATGAAGCTGGTTTGG-3′ | 396 | |
ATMintron61 | R 5′-CCTCTAGGATTGATTACTTG-3′ | ||
ATMexon62 | F 5′-TAAAGTTCACATTCTAACTGG-3′ | 267 | |
ATMexon62 | R 5′-GGTGCAAAGAACCATGC-3′ | ||
ATRexon46 | F 5′-GCTGCTGTCTTCTTTCACTTGTGACA-3′ | 333 | |
ATRexon46 | R 5′-CACCTTCATTCCCCGTAGTTCTTTCA-3′ |
