Figure 4
Immunohistochemistry for β-catenin in wound healing. A robust staining of mesenchymal cells is shown in the normally healing at 2 and 4 weeks, and in the hyperplastic wound compared to unwounded skin. At 2 weeks following injury (panel a) there is staining for β-catenin, located both in the cell cytoplasm and nucleus in the mesenchymal cells from the healing wound. A representative positively stained cell is labeled with an arrow. The staining is more intense at 4 weeks following injury (pannel b), and declines substantially at 12 weeks following the injury (panel c). The tissue from a hyperplastic wound continues to demonstrate elevated staining even more than 2 years following the initial injury (panel d). A section of normal skin is shown in panel e. The arrow points to positive staining of the epithelial cells. A control section, without the primary antibody, is shown from a 4-week-old wound in panel f.
