Figure 2 | Laboratory Investigation

Figure 2

From: Loss of blood group A antigen expression in bladder cancer caused by allelic loss and/or methylation of the ABO gene

Figure 2

Map of the 5′ CpG island of the ABO gene and result of BiPS analysis. (Top) CpG sites in the promoter region of the ABO gene are indicated by vertical lines. (Middle) The amplified DNA fragments from regions 1 to 7 are indicated. PCR primer set of each region was indicated by arrows. (Bottom) BiPS analysis of the ABO gene. Extra bands are indicated by asterisks. After SSCP analysis, the extra bands were excised from gels, reamplified by PCR, and sequenced. Results of the direct sequencing of the case with full methylation were shown in the lower panel. U: unmethylated control, M: methylated control, N: no methylation, P: partial methylation, F: full methylation, *extra band showing mobility shift.

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