Figure 4
A case of bladder cancer showing chimeric expression of the A antigen. DNA was extracted from areas showing positive or negative A antigen expression and subjected to blunt-end SSCP analysis using three single nucleotide polymorphic markers (ALDOB, ABO and VAV2) on 9q. The patient's genotype was A/O2. A allele was lost in the sample taken from the area showing negative A antigen expression, while O2 allele was lost in the sample taken from the area showing positive A antigen expression. Note that two polymorphic loci (ALDOB, 9q21.3 centromeric to the ABO locus and VAV2, 9q34.1 telomeric to the ABO locus) also showed LOH and suggested a large regional chromosome deletion, while the parental origin of the lost allele in these two loci was different between areas showing A-antigen positive or negative expression.
