Figure 5
From: Continuous real-time measurement of tumor necrosis factor-α converting enzyme activity on live cells
Linear regression analysis of the relationship between surface TACE expression on MonoMac-6 cells and FAM–TAMRA peptide cleavage. Different passages of the cells were analyzed for surface TACE expression using flow cytometry, expressed by mean of fluorescent intensity (MFI) of the total cell population with values of isotype control subtracted, and for enzyme activity using FAM–TAMRA peptide. R2 was 0.95 (n=7, P<0.01).
