Figure 1
Comparison of human ski, sno and fussel-18 sequences. (a) Complete cDNA sequence of fussel-18. The start codon is marked in bold, the stop codon in bold and italic. (b) Sequence alignment of human Ski, Sno and Fussel-18 amino-acid sequences. Conserved amino acids are in white letters on a black background. The underlined Cys2–His2 zinc binding module is a part of the SAND domain and conserved in all members of the Ski/Sno family. The peptide used to generate a polyclonal rabbit anti Fussel 18 antiserum is framed. (c) Homology between Ski, Sno and Fussel-18 on protein level shown as a homology tree. The tree was constructed using the program DNAman based on the alignment of the complete coding region. The length of each horizontal line is proportional to the degree of protein sequence divergence. (d) Schematic diagram of the structural organization. The conserved Ski homolog regions of Ski, Sno and Fussel-18 are shown. The amino-acid homology of this area is indicated in %.
