Figure 3

Fussel-18 expression in human tissues. Expression pattern of fussel-18 was verified by semiquantitative PCR from several human tissues. Expression of fussel-18 mRNA was detected in cerebellum and spinal cord and at very low levels in testis. Western blot analysis of Fussel-18 confirmed the expression of Fussel-18 in cerebellum. As a control for the specificity of the antibody we competed the binding of the antibody using 5 μg/ml peptide against Fussel-18. Two specific bands were detected about 30 and 60 kDa.