Figure 2

Northern blotting analysis (a) and RT-PCR (b) of VEGF mRNA tissue distribution in Tg rabbits. Rehybridization of the membrane with a β-actin probe showed that similar amounts of RNA had been loaded in each lane. Human VEGF expression was found only in Tg rabbit liver. The specific expression of hVEGF was confirmed by real-time RT-PCR (b).