Table 1 Antibodies used for staining of phyllodes tumors
Antibody | Source | Clone | Dilution | Pretreatment |
|---|---|---|---|---|
EGFR | Monosan | EGFR.113 | 1:20 | Steamer, citrate buffer, 35 min |
c-kit | DAKO | (polyclonal) | 1:100 | Steamer, citrate buffer, 35 min |
Ki-67 | DAKO | Mib-1 | 1:1000 | Steamer, citrate buffer, 35 min |
p53 | DAKO | DO-7 | 1:2000 | Steamer, citrate buffer, 35 min |
c-myc | Santa Cruz | 9E10 | 1:150 | Steamer, citrate buffer, 35 min |
p16INK4a | Neomarkers | 16P07 | 1:160 | EDTA buffer, 20 min, 97°C |
p21 | Oncogene | WAF1 | 1:10 | Citrate buffer, 20 min, 97°C |
p27Kip1 | BD Pharmingen | 57 | 1:1000 | Citrate buffer, 20 min, 97°C |
Cyclin A | Monosan | 6E6 | 1:100 | Citrate buffer, 20 min, 97°C |
Cyclin D1 | Novocastra | DCS-6 | 1:20 | EDTA buffer, 20 min, 97°C |
Cyclin E | Monosan | 13A3 | 1:200 | Citrate buffer, 20 min, 97°C |
