Figure 4
From: Treatment with granulocyte colony-stimulating factor ameliorates chronic heart failure
Expression of G-CSFR and activation of downstream signaling. (a) Western blot showing myocardial expression of G-CSFR, phospho-Akt, phospho-Stat5 and phospho-ERK. (b) Expression of G-CSFR quantified by densitometry. (c) Immunohistochemical analysis revealing the distributions of C-GSFR and MHC: G-CSFR, green; MHC, red; nuclei stained with Hoechst, blue; areas where G-CSR and MHC overlap, yellow. Right panels show the serial section incubated with the isotype negative controls, showing neither green nor red fluorescence. Bars, 20 μm. (d) Expression of phospho-Akt, phospho-Stat5 and phospho-ERK quantified by densitometry. *P<0.05.
