Figure 6
From: Treatment with granulocyte colony-stimulating factor ameliorates chronic heart failure
Expression of cardiac fibrosis-related proteins. (a) Western blot showing myocardial expression of MMP-2 and -9, TIMP-1 and -2, AT1 and TGF-β1. (b) Their quantification by densitometry. (c) Myocardial TNF-α content determined by ELISA. *P<0.05. (d) Immunohistochemistry for MMP-2 and -9, AT1, and TGF-β1 in infarcted (left panels) and noninfarcted (right panels) areas of hearts with 16-week-old MI. Immunohistochemical preparations for MMP-2 and MMP-9 shown here were obtained from the G-CSF-treated hearts, and those for AT1 and TGF-β1 were from the untreated hearts. Bars, 20 μm.
