Table 2 Morphometric analysis of (peri-)canalicular area of zone 2 hepatocytes from livers treated with DMSO (0.1%, v/v), TLCA (10?μmol/l), or TLCA (10?μmol/l)+TUDCA (25?μmol//l), each for 50?min

From: Tauroursodeoxycholic acid inserts the bile salt export pump into canalicular membranes of cholestatic rat liver

(n)

DMSO (control) (5)

TLCA (10?μmol/l) (4)

TLCA (10?μmol/l)+TUDCA (25?μmol/l) (5)

Arbitrary units (AU)

   

Pericanalicular zone (1?μm) volume

52.2±6.3

58.9±3.2

61.8±6.1*

Canalicular lumen volume

14.8±3.1

17.7±3.5

17.4±5.2

Canalicular membrane (without microvilli) surface area

11.0±2.4

9.8±2.5

10.7±1.3

Microvilli surface area

17.3±2.0

19.7±2.8

19.5±6.0

  1. Liver tissue was fixed immediately after perfusion. Morphometric analysis was performed by electronmicroscopy at a magnification of × 40?000 according to Weibel et al23 and Weibel.24 For experimental details, see also Methods.7 Results are given as mean±s.d. of 4–5 tissue samples in arbitrary units of volume or area, (each single data point represents a mean of six measurements per tissue sample).
  2. *P<0.05 vs control, unpaired two-tailed Student's t-test.
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