Figure 5
From: Biobanking of fresh frozen tissue: RNA is stable in nonfixed surgical specimens
RNA integrity of colon tissue under different transport conditions. Normal colon tissue was processed as outlined in Figure 1. Sample storage in saline was omitted in this experiment due to lack of available tissue. Purified RNA was analysed using microchip gel electrophoresis (Bioanalyzer) and the results are presented as electropherograms. The RNA of all samples (stored at RT, on ice or in RNAlater) and at all time points (0, 0.5, 1, 3, 6, 16 h) showed distinct ribosomal peaks indicating no significant degradation. Representative RNA samples purified with a Qiagen column are shown.
