Figure 6

Representative image of pancreatic MSCs differentiated into adipocytes (a, b), chondrocytes (c, d) and hepatocytes (e–h). ORO staining detects lipid droplets (a) and immunohistochemical staining for FABP4 (brown) is positive (b). Lipid droplets are red, positive immunoperoxidase stain is brown and hematoxylin counterstain is blue. Chondrocyte pellet is positive for alcian blue stain (c) and immunohistochemical staining for aggrecan is positive (d). Positive immunoperoxidase stain is brown and hematoxylin counterstain is blue. Scale bar represents 50 μm. Hepatogenic differentiation of pancreatic MSCs (e–h). Phase contrast demonstrates that after 16 days, pancreatic MSCs lose their fibroblast-like morphology (e) and assume a cuboidal morphology (f). Magnification × 40. Immunohistochemical staining for human serum albumin (g) and GATA 4 (h) is positive in cuboidal cells harvested after 27 days of differentiation. Positive immunoperoxidase stain is brown and hematoxylin counterstain is blue. Scale bar represents 50 μm.