Table 1 Primers used for PCR amplification
From: Expansion of mesenchymal stem cells from human pancreatic ductal epithelium
Gene | Size (bp) | Strand | Sequence 5′–3′ | GenBank |
|---|---|---|---|---|
Amylase | 300 | F | GAC TTT CCA GCA GTC CCA TA | |
| Â | Â | R | GTT TAC TTC CTG CAG GGA AC | Â |
Beta-2 microglobulin | 268 | F | CCA GCA GAG AAT GGA AAG TC | |
| Â | Â | R | GAT GCT GCT TAC ATG TCT CG | Â |
GATA 4 | 366 | F | CTC CTT CAG GCA GTG AGA GCC | |
| Â | Â | R | GGT CCG TGC AGG AAT TTG ACG | Â |
Glucagon | 221 | F | CCC AAG ATT TTG TGC AGT GGT T | |
| Â | Â | R | GCG GCC AAG TTC TTC AAC AAT | Â |
Insulin | 263 | F | GCA GCC TTT GTG AAC CAA CA | |
| Â | Â | R | GTT GCA GTA GTT CTC CAG GTG | Â |
Ngn3 | 313 | F | GGT AGA AAG GAT GAC GCC TC | |
| Â | Â | R | CCG AGT TGA GGT CGT GCA T | Â |
NeuroD | 439 | F | ATC CCA ACC CAC CAC CAA CC | |
| Â | Â | R | CAG CGG TGC CTG AGA AGA TT | Â |
Pax 4 | 496 | F | AGG AGG ACC AGG GAC TAC CGT | |
| Â | Â | R | TTT AGG TGG GGT GTC ACT CAG | Â |
PDX1 | 262 | F | CCC ATG GAT GAA GTC TAC C | |
| Â | Â | R | GTC CTC CTC CTT TTT CCA C | Â |
Tyrosine | 358 | F | TGA GCA GTC TGT CCA CTG CCT | |
Aminotransferase | Â | R | ATG TGA ATG AGG AGG ATC TGA G | Â |
