Figure 1 | Laboratory Investigation

Figure 1

From: Meth-DOP-PCR: an assay for the methylation profiling of trace amounts of DNA extracted from bodily fluids

Figure 1

The Meth-DOP-PCR assay. (a) Outline of a typical Meth-DOP-PCR experiment compared to direct MSP. The sequence of the DOP primer for native DNA and those for the modified DNA are indicated. N=A, C, G, T; H=A, C, T; D=A, G, T. (b) Requirement for the low-stringency amplification step. Lane A: 100 bp ladder. The modified DNA was subjected to Meth-DOP-PCR as described in Materials and methods (lane B) or omitting the enzyme only in the low stringency step (lane C).

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