Figure 3 | Laboratory Investigation

Figure 3

From: Meth-DOP-PCR: an assay for the methylation profiling of trace amounts of DNA extracted from bodily fluids

Figure 3

Fidelity of Meth-DOP-PCR. The methylation status of the ΔN-p73 promoter was determined by BRE before (B) and after (D/B) Meth-DOP-PCR. The DNA utilized in this analysis derived from U937 and ACN, two cell lines where this promoter is completely methylated and unmethylated, respectively. BRE analysis was performed with primer sets that do not discriminate between methylated and unmethylated sequence. The methylation level was determined by digestion with Rsa I, a restriction enzyme whose recognition sites is created by bisulfite modification in the methylated DNA only. U indicates the uncleaved amplification band corresponding to the non-methylated target, M is the band originated by restriction enzyme digestion.

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