Figure 1 | Laboratory Investigation

Figure 1

From: Hepatic stellate cells primed with cytokines upregulate inflammation in response to peptidoglycan or lipoteichoic acid

Figure 1

Characterization of telomerase-positive HSCs and expression of peptidoglycan and lipoteichoic acid receptors in primary human HSCs. (a) The expression of type I collagen, α-SMA, and fibulin-2 in a telomerase-positive human HSC cell line (hTERT) was assessed by Western blotting. JAR cell lysate was used as a positive control for fibulin-2. (b, c) TLR2 and CD14 mRNA expression was assessed by RT-PCR in telomerase-positive HSC cell line and primary human HSCs. (b) Total RNA was obtained from an activated human HSC cell line (hTERT) or from differentiated THP-1 cells. THP-1 cells were differentiated by treatment with 10 ng/ml of PMA for 18 h, and were used as a positive control. (c) Total RNA was obtained from quiescent human HSCs or in vitro culture-activated human HSCs (from normal liver), and in vivo activated human HSCs (from HCV-induced cirrhotic liver).

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