Figure 1 | Laboratory Investigation

Figure 1

From: Development and characterization of a cholangiocyte cell line from the PCK rat, an animal model of Autosomal Recessive Polycystic Kidney Disease

Figure 1

Development of the PCK-CCL. Bile duct explants were isolated by enzyme digestion from 2- to 3-month old PCK rats and placed on type I rat tail collagen supplemented with 10% Matrigel. Cells spread from these explants and proliferated forming cysts (a). The individual cysts were selected and sub-cultured; 5–7 days later they formed a monolayer (b) composed of epithelial cells. Magnifications: × 4 (a and b).

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