Figure 3 | Laboratory Investigation

Figure 3

From: Development and characterization of a cholangiocyte cell line from the PCK rat, an animal model of Autosomal Recessive Polycystic Kidney Disease

Figure 3

PCK-CCL growing on the top of collagen gel. Phase-contrast microscopy showed a tight monolayer formed by PCK-CCL grown in culture for 7–14 days (a). Tight junctions were present between adjacent cells as shown by expression of ZO-1 (b). Adhesion junctions were labeled using an antibody against β-catenin (c). Well-maintained polarity is indicated by numerous microvilli, appropriately developed tight (TJ) and desmosomal (D) junctions and basolaterally located nuclei as shown by transmission electron microscopy (d, e). Transepithelial electrical resistance of the PCK-CCL monolayers (circles) was measured during two weeks in culture. NRCs (boxes) were grown under identical conditions and served as a control (f; n=3 for each cell line). Scale bars 10 μm (b and c), magnification × 20 (a).

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