Figure 4

Scanning electron microscopy (a and c; scale bars: 2 μm) and immuno-fluorescence confocal microscopy (b and d; scale bars: 10 μm) of primary cilia in PCK-CCL (a and b) and NRCs (c and d) grown on a collagen gel for 10–14 days after confluence. Cilia were stained with ciliary marker acetylated a-tubulin (red; b and d) and nuclei were stained with DAPI (blue). Inset (a; scale bar 1 μm) shows single primary cilium at higher power.