Figure 1
From: DNA methylation-dependent silencing of CST6 in human breast cancer cell lines
CST6 expression in human breast cancer cell lines and normal mammary epithelial cells. Representative agarose gels of RT-PCR products are shown. (a) Differential expression of CST6 among breast cancer cell lines and two normal mammary epithelial cell lines (MCF10-2A and MCF12A). The CST6 RT-PCR product is 163 bp in size. β-Actin was utilized as a sample control. (b) Lane 1 corresponds to cDNA from the indicated untreated breast cancer cell lines; lanes 2 and 3 correspond to cells after 5-aza treatment or treatment withdrawal, respectively. β-Actin RNA was evenly expressed in all samples (data not shown).
