Figure 2
From: DNA methylation-dependent silencing of CST6 in human breast cancer cell lines
Quantitive real-time PCR analysis of CST6 in breast cancer cell lines. β-Actin was used to normalize gene expression levels for each cell line and differences in CST6 expression were determined using the comparative Ct method. CST6 gene expression for cell lines treated with 5-aza (black bars) and after treatment withdrawal (gray bars) are expressed as relative fold-change compared to control values (set at 1.0). Error bars reflect s.e.m. Values for gene expression that do not show error bars reflect data where the s.e.m. could not be depicted graphically. *Denotes statistical significance at P<0.05 compared to control values. **Denotes statistical significance at P<0.04 compared to 5-aza treatment values.
