Figure 5
From: DNA methylation-dependent silencing of CST6 in human breast cancer cell lines
Methylation analysis for individual CpG dinucleotides in CST6-positive and CST6-negative breast cancer cell lines. (a) Distribution of CpG dinucleotides proximal to the transcriptional start site in the promoter (0 to −1400 nucleotides) and exon 1 (0 to +294 nucleotides) of CST6 are depicted schematically. Vertical lines represent the relative position of individual CpG dinucleotides and the segmented horizontal lines (designated 1–4) indicate the location of individual CpG dinucleotides depicted in (b) (segment 1), (c) (segment 2), and (d) (segment 4). The results for CpGs in segment 3 are not shown. Representative CpG dinucleotides are indicated by lollipops corresponding to various segments of the promoter or exon 1 (+242 to −228). TMI values represent averages of the three CST6-positive and five CST6-negative breast cell lines: black circles correspond to 100% methylated CpGs, partially-filled circles correspond to >40% methylated CpGs, gray circles correspond to <40% methylated CpGs, and white circles correspond to unmethylated CpGs. †Denotes statistical significance at P<0.001; **Denotes statistical significance at P<0.01; and *Denotes statistical significance at P<0.03, when comparing individual CpG methylation to CST6 gene expression status. (b) CpGs 1–15 from segment 1 (−50 to −200) within the promoter region. (c) CpGs 16–31 from segment 2 (+50 to −50) spanning the transcriptional start site. (d) CpGs 43–55 from segment 4 (+242 to +138) within exon 1.
