Figure 4

(a) abcd2—mRNA in 3-month-old WT mouse. Strong to moderate signal with the antisense probe in medulla, and a weaker signal in surrounding cortex (top left and bottom). There is no hematoxylin counterstain. The sense probe was entirely negative (data not shown) × 100. (b) Weak signal in both medulla (arrows) and inner cortex (double arrow) of 3-month-old abcd2 KO. Anti-SOD2 X 100. Hematoxylin counterstain. (c) Extremely weak signal in outer cortex (arrow) of 3-month-old WT mouse. Anti-SOD2 × 100. Hematoxylin counterstain.