Figure 1

Effect of glucose on HPMC proliferation. Cells were grown into senescence in the presence of NG, HG, or equimolar M, as described in Materials and methods. (a) Kinetics of growth: the cumulative number of PD was assessed at each passage. The data were derived from 9 experiments with cells from different donors. The P-value refers to the impact of glucose on the difference between the curves as analyzed by two-way ANOVA. (b) The total number of PD achieved by HPMC grown into senescence under different conditions. The means of 11 experiments with cells from separate donors.