Figure 4
Effect of glucose on the expression of p21Waf1 and p27Kip1 by senescent HPMC. Cells were grown into senescence in the presence of either NG, HG, or equimolar M. (a) p21Waf1 activity was assessed by immunoassay in four experiments performed with cells from three different donors; (b) the percentage of cells staining positively for p27Kip1. The results of eight independent experiments with cells from different donors. (c) p27Kip1 mRNA expression by early- and late-passage HPMC as assessed by RT-PCR; a representative experiment of two performed. (d) Densitometric p27Kip1/β-actin ratios were expressed as a percentage of the value characterizing early-passage control cells.
