Figure 5

Effect of glucose on the expression of TGF-β1 by senescent HPMC. Cells were grown into senescence in the presence of either NG, HG, or equimolar M. (a) The mean TGF-β1 release by early- and late-passage HPMC. The data derived from 10 experiments with cells from separate donors. (b) TGF-β1 mRNA expression by early- and late-passage HPMC as assessed by RT-PCR; a representative experiment of two performed. (c) Densitometric TGF-β1/β-actin ratios were expressed as a percentage of the value characterizing early-passage control cells.