Figure 6
Effect of TGF-β1 on HPMC growth. (a) Serum-stimulated early-passage cells were incubated with increasing doses of TGF-β1 and the synthesis of DNA was assessed by 3H-thymidine incorporation. The data were derived from 3 independent experiments with the use of cells from different donors. Asterisks represent a significant difference compared with cells not exposed to TGF-β1. (b) Serum-stimulated early-passage HPMC were treated with 1?ng/ml of TGF-β1 for 72?h and then immunostained for p27Kip1 and counterstained with Mayer's hematoxylin. Magnification × 100. (c) Percentage of cells expressing p27Kip1 after the incubation in the presence or absence of TGF-β1. The means of three independent experiments with cells from different donors. (d) p27Kip mRNA expression in early-passage HPMC treated with 10?ng/ml of TGF-β1 for the time-periods indicated; a representative RT-PCR experiment of two performed. (e) Densitometric p27Kip/β-actin ratios were calculated for cells incubated in the presence (▪) or absence (□) of TGF-β1.
