Figure 7
Effect of TGF-β1 on protein synthesis by HPMC. (a) Early-passage cells were incubated for 48?h with increasing doses of TGF-β1 and the de novo protein synthesis was assessed by 3H-proline incorporation. The data were derived from three independent experiments with the use of cells from different donors. Asterisks represent a significant difference compared with cells not exposed to TGF-β1. (b) The release of fibronectin by early-passage cells stimulated with TGF-β1 for 48?h. The data were obtained from three experiments with cells from different donors. Asterisks represent a significant difference compared with cells not exposed to TGF-β1. (c) Fibronectin mRNA expression in early-passage HPMC treated with 10?ng/ml of TGF-β1 for the time-periods indicated; a representative RT-PCR experiment of two performed. (d) Densitometric fibronectin/β-actin ratios were calculated for cells incubated in the presence (▪) or absence (□) of TGF-β1.
