Figure 9

Effect of TGF-β1 blockade on HG-induced increase in SA-β-Gal activity. Early-passage cells were exposed to HG for 5 days in the presence of neutralizing anti-TGF-β1 antibody. The media were changed every 2 days. SA-β-Gal activity was measured in HPMC lysates by specific quantitative assay. The data represent the means of nine experiments with cells from seven different donors.