Table 1 Primer sequences and PCR conditions
Sequence | Product size (bp) | PCR cycles | Annealing temperature (°C) | Reference |
|---|---|---|---|---|
β-Actin | ||||
F: 5′-GGAGCAATGATCTTGATCTT-3′ | ||||
R: 5′-TGCTCACAGGCAAGGTGTAG-3′ | 204 | 30 | 55 | |
TGF-β1 | ||||
F: 5′-GGCAGTGGTTGAGCCGTGGA-3′ | ||||
R: 5′-TGTTGGACAGCTGCTCCACCT-3′ | 510 | 35 | 55 | |
Fibronectin | ||||
F: 5′-AGCCGCCACGTGCCAGGATTAC-3′ | ||||
R: 5′-CTTATGGGGGTGGCCGTTGTGG-3′ | 439 | 22 | 55 | |
P27 Kip1 | ||||
F: 5′-TCTGTAGTAGAACTCGGGCAA-3′ | ||||
R: 5′-AGTTCAAACGTGCGAGTGTC-3′ | 266 | 29 | 60 | |
