Figure 2 | Laboratory Investigation

Figure 2

From: Analysis of genetic events that modulate the oncogenic and growth suppressive activities of the PAX3-FKHR fusion oncoprotein

Figure 2

Effects of low level constitutive PAX3-FKHR expression on transformation and growth suppression in NIH3T3 cells. (a) Western blot analysis of PAX3-FKHR expression in pBabe- and pK1-transduced cells. Lanes (loaded with 40 μg cell extract) containing vector control (C) and PAX3-FKHR in pBabe and PAX3-FKHR in pK1-transduced cells are labeled on the top, and positions of PAX3-FKHR (PF) and actin loading control are labeled at the right. (b) Cell growth of NIH3T3 cells transduced with PAX3-FKHR in pBabe. Triplicate cultures of PAX3-FKHR or vector-transduced cells were counted at the indicated time points. The cell numbers were normalized to the first day counts and expressed as the mean (±s.d.) of triplicate assays. (c) Soft agar colony assay of NIH3T3 transduced with PAX3-FKHR in pBabe. PAX3-FKHR or vector-transduced cells were plated in soft agar and scored after three weeks. Three plates were counted for each construct and results were expressed as the mean (±s.d.).

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