Figure 4 | Laboratory Investigation

Figure 4

From: Analysis of genetic events that modulate the oncogenic and growth suppressive activities of the PAX3-FKHR fusion oncoprotein

Figure 4

Effect of overexpression of MDM2, CDK4, MYCN and SV40 T antigen on growth of NIH3T3 cells expressing high level inducible PAX3-FKHR construct. (a) Western blot analysis of PAX3-FKHR-ER (in pK1 vector), MDM2, CDK4, MYCN and SV40 T antigen. PAX3-FKHR-ER (PF-ER) and oncoproteins expressed in cells (40 μg cell extract/lane) are indicated on the top, and positions of corresponding proteins, including actin loading control are labeled at the right. (b) Growth of NIH3T3 transduced with high level PAX3-FKHR-ER construct and additional oncogenes. Triplicate cultures of each set of transduced cells were treated with the indicated concentration, and counted at the indicated time points. The cell numbers were normalized to the first day numbers and expressed as the mean (±s.d.) of triplicate assays.

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