Figure 5

Growth status of NIH3T3 cells transformed by PAX3-FKHR. The original PAX3-FKHR-ER (from pK1) transduced population and cells from two transformed subclones (clones 8 and 12) derived from soft agar colonies were seeded in six-well plates. Triplicate cultures of each set of cells were treated with the indicated TMF concentration, and counted using trypan blue exclusion at the indicated time points. The cell numbers were normalized to the first day counts and expressed as the mean (±s.d.) of triplicate assays.