Figure 10
In vitro experiments (Protocol 3). (a) Confocal micrographs showing the atrophic degeneration of cardiomyocytes exposed to DOX and its prevention by G-CSF. Bars, 10 μm. Graph shows the dose-dependent effect of G-CSF on the size of cardiomyocytes exposed to DOX. (b and c) Western analysis of the effect of G-CSF on the DOX-mediated reduction in GATA-4 and MHC expression (b) and increase in COX-2 expression (c) in cultured cardiomyocytes. Bars are means±s.e.m.; *P<0.05 vs the untreated sham group (DOX (−) and G-CSF (−)); #P<0.05 vs the untreated DOX group (DOX (+) and G-CSF (−)).
