Figure 7
(a) Western (left) and immunohistochemical (right) analyses of the effect of G-CSF on G-CSFR expression in heart. Bars, 20 μm. (b) Western analysis of the downstream targets of G-CSFR signaling. The degree of activation of the PI3K/Akt, Jak/STAT and ERK/MAPK signaling pathways was assessed by determining the levels of the phosphorylated forms of Akt, STAT3 and ERK (p-Akt, p-STAT3 and p-ERK, respectively). Bars are means±s.e.m.; *P<0.05 vs the untreated sham group (DOX (−) and G-CSF (−)); #P<0.05 vs the untreated DOX group (DOX (+) and G-CSF (−)).
