Figure 9
(a) Graphs showing the number of migrated bone marrow-derived GFP-positive cells in hearts and the percentage of myocardial CD45-positive cells among those originating from bone marrow-derived cells. (b) Confocal micrographs showing double immunofluorescent labeling of GFP with MHC, CD45, Flk-1 or α-SMA. GFP positivity (green fluorescence) overlapped with CD45-positive leukocytes (red fluorescence), but not with cardiomyocytes, endothelial cells or vascular smooth muscle cells. When the primary antibodies were substituted with the control IgG, no fluorescence was noted of GFP or the cell specific proteins, confirming specificities of staining (the most right panels). Bars, 20 μm.
