Figure 1 | Laboratory Investigation

Figure 1

From: The interruption of the PDGF and EGF signaling pathways by curcumin stimulates gene expression of PPARγ in rat activated hepatic stellate cell in vitro

Figure 1

Exogenous EGF or PDGF dose-dependently reduces gene expression of PPARγ in activated HSC in vitro. After the treatment with or without curcumin in serum-free media for 24 h, passaged HSC were stimulated with PDGF or EGF at indicated doses in serum-free media for an additional 24 h. Total RNA or protein extracts were prepared from the cells for real-time PCR (a and c) (n=3), or Western blotting analyses (b and d) (n=3). β-Actin was used as an internal invariable control for (b and d). The level of PPARγ was densitometrically normalized with β-actin. The numbers beneath the blots were fold changes in the densities of PPARγ bands compared to the control without treatment in the blot (n=3). Because of the limited space, standard deviations were not presented. *P<0.05 vs cells with no treatment (The first black column on the left). **P<0.05 vs cells with only EGE or PDGF at the same dose.

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