Figure 3 | Laboratory Investigation

Figure 3

From: The interruption of the PDGF and EGF signaling pathways by curcumin stimulates gene expression of PPARγ in rat activated hepatic stellate cell in vitro

Figure 3

Curcumin blocks the activation of the ERK, JNK and PI-3K/AKT signaling pathways in activated HSC in vitro. After serum-starvation for 48 h, HSC were pre-treated with curcumin for 30 min before the stimulation with FBS (10%) for an additional 20 min. Whole cell extracts were prepared for Western blotting analyses. Representatives were shown from three independent experiments. (a) Curcumin reduces the level of phosphorylatetd ERK1/2 (p-ERK1/2). Total ERK1/2 was used as an internal control. (b) Curcumin reduces the level of phosphorylatetd JNK1/2 (p-JNK1/2). Total JNK1/2 was used as an internal control for equal loading. (c) Curcumin reduces the level of phosphorylatetd AKT (p-AKTser473 and p-AKTthr308). Total AKT was used as an internal control.

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