Figure 5 | Laboratory Investigation

Figure 5

From: The interruption of the PDGF and EGF signaling pathways by curcumin stimulates gene expression of PPARγ in rat activated hepatic stellate cell in vitro

Figure 5

The inhibition of ERK, JNK or PI-3K/AKT activity stimulates gene expression of PPARγ in activated HSC. With or without transfection, serum-starved HSC were respectively pretreated with or without LY294002 (a PI-3K inhibitor), SP600125 (a JNK inhibitor), or PD98059 (a MEK inhibitor) at indicated doses, or with curcumin at 20 μM, for 30 min before the stimulation with FBS (10%) for an additional 24 h. (a) Luciferase assays of cells transfected with pPPARγ-Luc, containing a fragment of PPARγ gene promoter in a luciferase reporter plasmid. Luciferase activities were expressed as relative units after β-galactosidase normalization (n≥6). The floating schema denotes the pPPARγ-Luc luciferase reporter construct in use and the application of an inhibitor to the system. *P<0.05 vs cells with no treatment (the second columns on the left). (b) Real-time PCR analyses of PPARγ mRNA (n=3). *P<0.05 vs cells with no treatment (the second columns on the left); (c) Western blotting analyses of PPARγ. Representatives were shown here from three independent experiments.

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