Figure 7 | Laboratory Investigation

Figure 7

From: The interruption of the PDGF and EGF signaling pathways by curcumin stimulates gene expression of PPARγ in rat activated hepatic stellate cell in vitro

Figure 7

The blockade of the PI-3K/AKT, ERK, and/or JNK signaling pathways results in the induction of apoptosis, which is partially eliminated by the inhibition of PPARγ activation. Serum-starved HSC were treated with or without curcumin (20 μM), or LY294002 (LY) (10 μM), or PD98059 (PD) (10 μM), or SP600125 (SP) (10 μM) for 24 h in the presence or absence of the PPARγ antagonist PD68235 (PD′) at 20 μM. Apoptotic HSC were detected by DeadEnd™ Fluorometric TUNEL System (Promega) and viewed using a fluorescent microscope. Nuclei of apoptotic cells were detected and labeled with green fluorescence indicated by arrows. Nuclei of HSC were stained in blue with DAPI. Representative views (n=3) from each treatment were presented (original × 40).

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