Figure 11

Measurement of the phosphorylation of (a) PKA, (b) ERK1/2 and (c) Elk-1 in purified BDL cholangiocytes treated in vitro with 0.2% BSA (basal) or PAMH (10 μM). (a) In vitro, RAMH induced a decrease in PKA phosphorylation in purified BDL cholangiocytes compared to BDL cholangiocytes treated with BSA. Data are mean±s.e.m. of three experiments. ^*P<0.05 vs corresponding basal value. (b) RAMH decreased the phosphorylation of ERK1/2 (b) and Elk-1 (c) compared to cholangiocytes treated with BSA. Data are mean±s.e.m. of three experiments. ^*P<0.05 vs corresponding basal value.