Figure 2 | Laboratory Investigation

Figure 2

From: H3 histamine receptor agonist inhibits biliary growth of BDL rats by downregulation of the cAMP-dependent PKA/ERK1/2/ELK-1 pathway

Figure 2

Immunofluorescence for H3R in frozen liver sections from normal and 1 week BDL rats. By immunofluorescence in liver sections, there was cytoplasmic and membranous staining (arrows) for H3R in cholangiocytes from normal and BDL rats. By immunofluorescence, H3R immunoreactivity (red) was colocalized with CK-19 immunoreactivity (green; indicated by arrows) demonstrating cholangiocyte expression (scale bar=20 μm); sections were counterstained with DAPI. H3R were also expressed by hepatocytes from normal and BDL rats. No immunofluorescent reaction was observed when normal or BDL liver sections were incubated with non-immune serum.

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