Figure 3 | Laboratory Investigation

Figure 3

From: H3 histamine receptor agonist inhibits biliary growth of BDL rats by downregulation of the cAMP-dependent PKA/ERK1/2/ELK-1 pathway

Figure 3

Immunofluorescence (a, top panel) for H3R in cytospin smears of isolated cholangiocytes, and real-time PCR (b, bottom panel) for H3R mRNA in cholangiocyte RNA (0.75 μg) from normal and 1 week BDL rats. By immunofluorescence (a, top panel), purified cholangiocytes from both normal and BDL rats express H3R. Original magnification, × 40. By real-time PCR (b, bottom panel), we found that (i) normal cholangiocytes express H3R mRNA (expressed as ratio to GAPDH mRNA); and (ii) the relative expression of H3R mRNA (expressed as ratio to GAPDH mRNA) significantly increased in cholangiocytes from BDL rats. Data are mean±s.e.m. of three experiments. *P<0.05 vs relative expression of H3R mRNA of normal cholangiocytes.

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