Figure 9

(a) Chronic administration of RAMH to BDL rats decreased the phosphorylation of Elk-1 (expressed as ratio to total Elk-1 protein expression) in cholangiocytes compared to cholangiocytes from NaCl treated rats. Data are mean±s.e.m. of three experiments. ^*P<0.05 vs corresponding value from BDL rats treated with NaCl. (b) Measurement of PCNA protein expression in purified BDL cholangiocytes treated in vitro with 0.2% BSA (basal) or RAMH (10 μM) with 0.2% BSA for 3 h at 37°C. In vitro stimulation of purified BDL cholangiocytes with RAMH induced a significant decrease in PCNA protein expression compared to cholangiocytes treated with 0.2% BSA. Data are mean±s.e.m. of eight experiments. ^*P<0.05 vs corresponding basal value.