Figure 2

Increase of CD4⁺CD25⁺ and decrease of CD4⁺ lymphocytes in cultured PBMCs with liver cancer cells by transwell. (a) Mean CD4⁺CD25⁺ frequency in CD4⁺ population from PBMCs before and after co-culture with Huh7, HepG2, and Hclone5 cells (n=8). (b) A representative flow cytometric data showing the frequency of CD4⁺ lymphocytes (CD4⁺CD3⁺) and CD4⁺CD25⁺ T cells in the PBMCs from a normal healthy control prior to co-culture with Huh7 (no culture), after culture with medium alone (medium), after culture with normal human hepatocytes (normal) or after culture with Huh7 cells (Huh7).