Figure 3 | Laboratory Investigation

Figure 3

From: Hepatocellular carcinoma cell supernatants increase expansion and function of CD4+CD25+ regulatory T cells

Figure 3

Phenotype analysis. Representative plots of PBMCs before and after co-culture with Huh7 cells. (a) Extracellular markers. (b) Intracellular markers. The degree of staining for the extracellular and intracellular markers for the control cultures (medium alone and normal hepatocytes) were statistically equivalent to the level of staining on the PBMCs before co-culture with Huh7 cells (data not shown) when labeled with antibodies for CD3, CD4, CD25, CD69, CD62L, CD45RA, CD45RO, GITR, CTLA-4, Ki67, FOXP3, granzyme A, granzyme B, and isotypes. Profiles were generated by gating on the CD25 high cells.

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