Figure 4

FOXP3 expression in PBMCs. Relative quantity of FOXP3 mRNA by real-time PCR using the ΔΔCt method (n=8) in PBMCs before and after culture with Huh7 cells in transwells for 48 h (a); and (b) after treatment with Huh7 supernatants at a 25 and 5% concentration.