Figure 6
Supernatants from Huh7 cells inhibit T-cell proliferation and induce CD4+ T-cell apoptosis. PBMCs were cultured with supernatants from various liver cancer cell lines (Huh7, Hep G2, Hep3B) and controls (medium alone, normal hepatocytes) for 3 days to assess their ability to proliferate in response to stimulation with anti-CD3 (10 μg/ml) by [3H]thymidine incorporation (a). PBMCs were cultured with different dilutions of Huh7 supernatants for 3 days in response to stimulation with anti-CD3 (10 ìg/ml) by [3H]thymidine incorporation (b). Proliferation data are expressed as mean±s.d. (*P<0.05 vs control). PBMC were co-cultured with Huh7 cells for 48 h by transwell, collected, and stained with Annexin V and PI to evaluate for apoptosis. Representative dot plots for CD4+ lymphocytes apoptosis are shown (c).
